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10.1074/jbc.274.30.21276 75

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Calcitriol is found in the kidneys (to increase calcium reabsorption from the glomerular filter), small intestine (to stimulate calcium absorption), and other tissues

The protocol notes reflect standard colorimetric assay discipline: do not mix or substitute reagents from other kit lots

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A dysregulated gut microbiome can lead to increased intestinal permeability (often called leaky gut'), which allows particles to enter the bloodstream and potentially trigger autoimmune responses, including those targeting the thyroid gland