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Upon 15 min incubation on ice, cell lysates were cleared by centrifugation for 15 min at 10,000 g and subjected to SDS-PAGE and Western blot analysis

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Collectively, the in vitro results confirmed that iron overload caused the aberrant miRNA components in exosomes, which then interfered with mouse oocyte maturation by affecting the cell cycle, oocyte meiosis, and ferroptosis signaling pathway, while deferoxamine mesylate and VITE can effectively improve this situation

TPEN also inhibited the secretion of both chemotactic IL-8 and anti-inflammatory cytokine IL-1ra in response to LPS stimulation [12]

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