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For example, elongated particles with larger contact surfaces tend to be less effective at lung targeting, as their shape promotes stronger van der Waals forces and aggregation 131

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They do not address underlying metabolic dysfunction and stop working when discontinued

Due to the loose adhesion of EpCAM compared to other CAMs, increased expression of EpCAM is associated with invasion, metastasis, and poor prognosis in many cancers, including basal-like and luminal B BCs [185, 187, 188]

J Nanopart Res 12:23132333 Almezgagi M, Zhang Y, Hezam K, Shamsan E, Gamah M, Al-Shaebi F et al (2020) Diacerein: recent insight into pharmacological activities and molecular pathways

Moreover, viral vector gene transfer has been tested pre-clinically and is more promising because it provides a more efficient transcription transfer than non-viral methods