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At the end of the permeability assay midbrain organoids were removed from the bottom of acceptor compartments, homogenized in DW containing Triton X-100 (10 mg/ml)

Thangaratnarajah C, Dinger K, Vohlen C, Klaudt C, Nawabi J, Lopez Garcia E, Kwapiszewska G, Dobner J, Nusken KD, van Koningsbruggen-Rietschel S, et al

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After a 2-week incubation period, the assays were terminated by fixing the cells in a methanol solution for 10 min, followed by staining with a 0.5% crystal violet solution for 30 min

The critical role of oxidative stress in sarcopenic obesity