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When deficiency is suspected based on clinical features (fatigue, paraesthesia, cognitive changes) or risk factors (vegan diet, gastric surgery, certain medications), serum B12 measurement guides appropriate intervention

Notably, RT-qPCR analysis showed a sharp increase in OCT4 mRNA expression, a marker of pluripotent stem cells and essential for TIC self-renewal [33], in the colonies formed in 3D gels relative to 2D culture conditions (Fig

Xu Q, et al

It's a reminder that biology often operates through subtle interactions between seemingly simple components, creating emergent properties that exceed the sum of their parts

PMID: 31389309