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Enzymatic treatment included 40U DNase-I, 1000 g/ml Amylase, 500 g/ml Proteinase K and a combination of 40U DNase-I + 4 g/mL amikacin or 40U DNase-I + 30 mM GSH

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We used ANCOVA analysis with adjustment for age, sex, ethnicity and smoking status to observe differences among the three groups

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Despite the extensive and multidisciplinary research during recent years, and the growing evidence on the anatomical and physiological lines of mucosal defense, the functional aspects of protection and healing still need to be better understood