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Schuetz et al

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Bacterial pellets were resuspended in GST-binding buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA) containing protease inhibitors

The difference in absorbance recorded at 412 nm before and 6 min after the addition of nicotinamide adenine dinucleotide phosphate in the presence of glutathione reductase was utilized to calculate the amount of total glutathione