For fluorescence microscopy studies, HL-60 cells and HepG2 cells (seeding densities 1 10 4 cells/well and 8 10 4 cells/well, respectively) were placed in serum-free medium to facilitate their adhesion to coverslips and cultured on lysine-coated 8-well chamber slides (IBIDI) following the procedure detailed by 2.8 Cell surface binding, endocytosis, and downregulation of TfRs For surface binding and endocytosis of AF-labeled proteins, the cells were rinsed twice with ice-cold HEPES-buffered, serum-free media, pH 7.4, and incubated for 1 h on ice with either 500 nM hemeAF546HPX, hemeAF546HPX, or AF488 holo-human Tf
In GCLM KO mouse liver, constitutive activation of AMPK was observed as measured by levels of phosphorylated AMPK and phosphorylated ACC
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The peptide prevented hyperglycemia-induced cellular dysfunction by maintaining redox balance and protecting against oxidative damage to DNA, proteins, and lipid structures
J Biol Chem 273:2991529922 Coblenz A, Wolf K (1994) The role of glutathione biosynthesis in heavy metal resistance in the fission yeast Schizosaccharomyces pombe
Moreover, the duration and patterns of ethanol exposure in animal studies may not accurately reflect the chronic alcohol consumption behaviors observed in human populations