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The dye was extracted from fixed and stained cells with 0.1% SDS/PBS and the absorbance quantified at 660 nm (Dynatech Laboratories, MR5000)

This indicates that the expression of these genes is responsive to reduced (h)GSH concentrations

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HUVECs and NIH 3T3 cells in Roswell Park Memorial Institute (RPMI) 1640 and HaCaT in Dulbeccos Minimum Essential Medium (DMEM)/F-12 medium were cultured in the indicated media supplemented with 10% fetal bovine serum (FBS) and maintained at 37C in a humidified environment with 5% CO 2