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In mass spectrometry, the ionization method was an ESI source, with an ion source temperature of 600C and an ion source voltage of either -4500 V or 5500 V

RNA concentration was measured using NanoDrop 2000 and RNA integrity was assessed by agilent Bioanalyzer 2100

We also found that post-MI hearts exhibited a significant increase in the expression of the necroptotic related proteins pRIPK3/tRIPK3 and pMLKL/tMLKL, which indicated myocardial necroptosis after MI

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Reconstitution, Dosage and Cycling, Research Only BAC Water 3ml is supplied as a research-use reconstitution medium

Mol Cell Biol 31 , 11211133, (2011)