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Under physiological conditions, they contribute to bloodbrain barrier (BBB) integrity, ion and fluid balance through aquaporin-4 (AQP-4) channels, synaptic modulation via glutamate transporters (excitatory amino acid transporters [EAATs], including glutamate transporter-1 [GLT-1] and glutamateaspartate transporter [GLAST]), and metabolic support through lactate shuttling (Yang et al., 2020)

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Additional considerations include the comfort of the subject