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We propagated each of these USC lines for between 15 and 30 passages, then differentiated them on transwells and characterized each cell line by microscopy after 23 weeks of culture

Expert Consensus on Injection Technique and Area-Specific Recommendations for the Hyaluronic Acid Dermal Filler VYC-12L to Treat Fine Cutaneous Lines

doi: 10.1038/s41385018-00218 32 HerbMSchrammM

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For disease evaluation caused by DC3000, the DC3000 strain was resuspended in 10 mM MgCl 2 at OD 600 = 0.0005