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Plasmid Construction and Transfection Assay Full-length GSTA1 and CPLX2 were generated using standard PCR methods and primers ( GSTA1 -His-F: CGGGATCCATGGCAGAGAAGCCCAAGCT, GSTA1 -His-R: CGGAATTCTTAATGATGATGATGATGATGAAACCTGAAAATCTTCC, CPLX2 -His-F: CGGGATCCATGGACTTCGTCATGAAGCAGG

Figure 7 shows the productivity of -EC in the repeated five-batch reactions using NsPCSs immobilized on the cellulose sponge and MCC

10.1007/s11064-008-9782-x 15

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