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In 28-month-old mice, roughly equivalent to elderly humans, three weeks of GHK-Cu administration produced significantly faster maze navigation with evidence of decreased brain inflammation and increased histone deacetylase 2 labeling, an indicator of epigenetic pathway activation

Ouyang, C

Chekalina NI, Shut SV, Trybrat TA, Burmak YH, Petrov YY, Manusha YI, et al

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On day 2, cells were transfected with either scrambled control siRNA (Silencer Select Negative Control #1 siRNA, 4390843, Thermo Fisher Scientific) or siRNA targeting the glucocorticoid receptor (siGR) (s67066, Thermo Fisher Scientific) using Lipofectamine-mediated transfection