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Essential Equipment and Materials Required Equipment: Sterile glass vials or low-binding microcentrifuge tubes Calibrated micropipettes (10-1000 L range) Sterile filter tips Vortex mixer or gentle rocker Analytical balance (0.1 mg precision) Reconstitution Solvents: Sterile water (molecular biology grade) Bacteriostatic water (contains 0.9% benzyl alcohol as preservative) Phosphate buffered saline (PBS, pH 7.4) Dilute acetic acid solution (0.1% v/v) DMSO (for highly hydrophobic peptides) Optional Materials: Sonicator bath pH meter 0.22 m sterile filters All materials must be sterile and free from endotoxins for cell culture applications
Adding up to 200 mM of strong Cu(II) chelators (DTPA, EDTA) at pH 7.4 did not decrease the metal content in CuCP peak even after 100 min incubation with 200 mM EDTA or 21 h incubation with 100 mM DTPA (Supplementary Information, Figure S1a)
So, you need to make sure youre getting enough iron, but not building up too much iron in your body
The vitamin B12 injection is given as an intramuscular injection in the upper arm (Deltoid muscle)
