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Gene expression profiling by quantitative reverse transcription-PCR Total RNA isolation from barley leaves was performed with GeneMATRIX Universal RNA Purification Kit (EURx, Gdask, Poland) according to the manufacturer's instructions (with additional treatment of RNase-free DNase I)

34 Quantification of GSH and Cr in phantoms by LCModel processing with our specialized basis set showed excellent correlation with known concentrations of these metabolites

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In the following sections, we will discuss: (1) -cell heterogeneity as revealed by scRNA-seq

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