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This becomes particularly important for athletes following rigorous training schedules or participating in multi-day events

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An acceptable MS certificate for IGF-1 LR3 should show: Observed molecular weight matching theoretical mass within acceptable tolerance (~0.1%) No major masses corresponding to truncated sequences or non-native analogs Deconvolution data showing the correct multiply-charged ion series Together, HPLC + MS provides a two-dimensional confirmation: the correct compound (MS) at sufficient abundance (HPLC)

Blood glucose levels after glucose administration were recorded

Role of vascular hormones and factors