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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

Matern Child Nutr 2014;10:22633

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These cells exhibited a loss of myofibroblastic features and are typically situated at a distance from cancer cells [213]

Take natural child development into account and recognize that all children go through stages during which they gain new skills or may focus less on other skills previously acquired

National Organization for Rare Disorders