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For further differentiation into HBs, a combined protocol of BMP4, IWR-1, and TGF inhibitor SB431542 eliciting hepatic markers, including fetoprotein (AFP), albumin (ALB), and prospero homeobox 1 (PROX1) was established (Touboul et al., 2016)

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any discrepancy suggests synthesis failure or contamination

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