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NFT or DEM treatment not only resulted in the translocation of a fraction of the Nrf2 into the nucleus but also showed dispersed localization in the cytosol

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The reasons for these failures often include short in vivo half-life, chemical instability, limited cellular uptake, poor bioavailability, lack of targeted delivery to specific tissues or (sub)cellular compartments, a narrow therapeutic index, and toxicity

Future studies including a broader age group and using specific behavioral assessments and scales are warranted

For nuclei with spin I = 1/2, where the magnetic moment aligns or opposes the magnetic field, resulting in detectable and observable energy differences, NMR is especially helpful