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The remaining 23 mL of each culture was quickly filtered under vacuum (Air Cadet vacuum/pressure station, Thermo), immediately washed with cold saline solution (9.5 g L 1 NaCl at 12 C) and quenched in cold methanol/water (1:1 v/v) at 30 C as described by Smart et al

Phosphatidylcholine supplementation in pregnant women consuming moderate-choline diets does not enhance infant cognitive function: A randomized, double-blind, placebo-controlled trial

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However, the simultaneous modification of numerous proteins under differing physiological and pathological conditions complicates the isolated analysis of O-GlcNAcylation on individual proteins