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In the mature DPP1 molecule, the exclusion domain binds the catalytic domain through non-covalent interactions and restricts substrate access to regions upstream of the S2 subsite via steric hindrance, determining DPP1s unique dipeptidyl peptidase activity ( The DPP1 active site structure reflects the molecular basis for its broad substrate specificity

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The assembly progression showed that both nonassembled compounds ( 1 , f = 1.1 ns and 2 , f = 2.0 ns) had a longer f than their assemblies ( 1 NF , f = 0.3 ns and 2 NP , f = 0.5 ns)

[PubMed: 6026079] 475

Jessup, H.K

10.1186/s13287-019-1253-6 19 ChenC.WangJ.SunM.LiJ.WangH