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If your model shows no effect, verify: (1) compound purity and correct amino acid sequence through independent analysis, (2) administration route appropriate for the peptide's pharmacokinetics, (3) dosing within the range established in analogous studies, and (4) treatment initiation timed to the injury or pathology phase the peptide's mechanism actually targets
Kerr, E
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Reverse transcription was performed to obtain cDNA in a 20 L reverse transcription system, consisting of 1 L random primers, 1 L Oligo dT Primer, 4 L Reverse Transcription buffer, and 1 IU/mL PrimeScriptTEMRT Enzyme Mix I (TaKaRa, Dalian, China)
