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How Many Glutathione Sessions Are Required

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The lysate was subsequently clarified by centrifugation, bound in-batch to glutathione sepharose 4B resin (Cytiva), washed extensively with sonication buffer, and subjected to on-resin TEV protease cleavage in 25 mM Tris pH 7.5, 75 mM NaCl overnight at 4 C

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All classes obtained after 3D classification and refinement showed the best results in terms of overall resolution and appearance of electron density