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Culturing was performed in RPMI 1640 medium enriched with 10% fetal bovine serum, maintained at 37C in a humidified incubator with an atmosphere of 5% CO 2 and 95% air

Following the clotting period, all specimens were centrifuged for 10 min at 1,300 RCF

pH sensitivity To confer pH sensitivity, lipids with ionizable group, such as 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), are incorporated due to their fusogenic properties under acidic conditions

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The enclosed networks of tubes were photographed 12 hours later using Canon PowerShot A640 camera on Zeiss inverted microscope with 100 magnification