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Currently, research on the anticancer activity of plant polysaccharides primarily focuses on GPX4-dependent ferroptosis pathways, while GPX4-independent signaling pathways, such as FSP1/CoQ10, DHODH and GCH1/BH4 pathways, remain unexplored

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Following the dissociation, TrypLE Express was inactivated by adding 9 mL of ice-cold Advanced DMEM/F12++ containing 10 M Y-27632 and the cells were collected by centrifugation at 400 g for 5 min at 4 C

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