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This phosphomimetic, S114D mutant, in NHR-49::YFP strongly favored a cytoplasmic localization, which was dramatically different from the wild-type and more so from the phospho-dead S114A mutant (Fig

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In order to obtain the fluorescence intensity values for a fully oxidized and reduced probe, following the run each well was sequentially treated with N,N,N,N-tetramethylazodicarboxamide (diamide) to a final concentration of 2 mM and DTT to a final concentration of 10 mM

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