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The protein bands were visualized by using an enhanced chemiluminescence (ECL) HRP kit (fluorescence detection (FD) bio-Femto (Yeasen, Shanghai, China)

4,5 Hcy was identified in 1932 by Du Vigneaud and later recognized as an intermediate in the methionine metabolic pathway, forming cysteine through the transsulfuration pathway

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Further, a causeeffect relationship has been reported between genes coding for nitrogen metabolizing enzymes such as glutamate dehydrogenases (GDH EC 1.4.1.3), ornithine decarboxylases (ODC EC 4.1.1.17), and chitin biosynthetic enzymes such as chitin synthases (CHS EC 2.4.1.16)

63 , e12410 (2017)

Supplementary material The Supplementary Material for this article can be found online at References 1 TownsendDMTewKD