Target proteins were detected with the enhanced chemiluminescence (ECL) system and quantified using a densitometric image analyzer with Image-Pro Plus 4.5 software (Media Cybernetics Inc., MD)
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Differential regulation of GHRH-receptor and GHS-receptor expression by long-term in vitro treatment of ovine pituitary cells with GHRP-2 and GHRH
We attempt to substitute the lack of direct conclusive evidence (i.e., a chemical produced within the neuron or present in it as a precursor molecule, released eliciting a response on the receptor on the target cells on neurons and being removed from the site of action once its signaling role is complete)