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doi: 10.1128/aac.02429-19 316 PetrikM.FranssenG

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When the energy charge is low, CoASH is not acetylated, therefore, pyruvate carboxylase is inactive, and pyruvate is preferentially oxidized to acetyl-CoA via the PDHc

After adding 0.6 ml of 10% TCA, 0.6 ml of 44%, orthophosphoric acid, 0.6 ml 4% , -dipyridyl 70% ethanol, and 0.3% (w/v) FeCl3 reagents color was developed in both reaction mixtures