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Methylation of DNA is catalyzed by DNA methyltransferases (DNMTs)

Glutathione is one of the most critical antioxidants in the body, helping to neutralize free radicals that can damage cells and tissues

Endogenous glucocorticoid secretion follows a conserved circadian pattern across species, with diurnal organisms (e.g., humans) showing peak concentrations during active phases (morning) and nocturnal species (e.g., rodents) displaying maximal levels during their active period (evening) (126).In humans, peak circadian cortisol secretion (approximately 06:0010:00h) (127) temporally coincides with maximal expression of tight junction proteins (occludin, claudin-5), and optimal BBB integrity

Images were captured using a confocal laser-scanning microscope to assess NF-B p65 nuclear translocation