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Images were acquired on a Zeiss Axio Observer 7 and cells were hand-counted in a strictly blinded fashion

Briefly, mice were anaesthetised with isoflurane (3% induction, 1.52% maintenance) given at 1 L/min O2 using TEC 3 Isoflurane Vaporiser (98723N, +Mediquip LTD)

GSH can then be synthesized de novo in a two-step ATP-requiring enzymatic process utilizing glutamate cysteine ligase (GCL) and glutathione synthetase (GS) (Figure 2) [83,87]

Clinical practice guidelines for chronic obstructive pulmonary disease: A review and comparison of current resources

Left ventricular hypertrophy is a hallmark of CKD and the main factors underlying its development include insulin resistance, accumulation of endogenous cardiotonic steroids and uraemic toxins, vitamin D deficiency, hyperphosphataemia and haemodynamic overload, which induces mechanical stress

40 and expressed as units (mol of dianisidine oxidized per minute) per mg of protein