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The suspension was freeze-thawed 3x at 80C, sonicated 3x 15 s then spun at 12 k RPM for 30 min to pellet the insoluble material

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Studies have demonstrated that stability can be significantly enhanced by extensive chemical modification at the 2 position of nucleotide sugars and by replacing the phosphodiester bond with a thiophosphate bond

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doi:10.1016/S0888-7543(05)80373-8

Following the completion of all animal tests, the apparatus should be promptly cleaned of any feces and urine