The enzymatic activities of -glutamylcysteine synthetase and gluthione synthetase were determined using the method described previously with a slight modification 43
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This basal medium was then augmented with a specific cocktail of small molecules: 3 M or 1 M CHIR99021 (CHIR), IDE1, 2 M Ly294002 (Ly), and 0.75 M PD0332991 (PD)