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While slower than at 37 C, metabolism at 4 C favors the intracellular acidification, in turn inhibiting pH-sensitive enzymes at rate-limiting steps of glycolysis, the Rapoport-Luebering shunt, and the pentose phosphate pathway (PPP) 11,12

doi: 10.3389/fmed.2022.1033936

Determination of the total and reduced forms of blood plasma aminothiols (GSH, cysteine, and homocysteine) was carried out by liquid chromatography (Acquity UPLC H-class system, Waters, Milford, Massachusetts, USA) as described previously [13]

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