Briefly, Huh7 cells were lysed in buffer (150 mM KCl, 25 mM Tris pH 7.4, 5 mM EDTA, 0.5 mM DTT, 0.5% NP-40, 9 g ml 1 leupeptin, 9 g ml 1 pepstatin, 10 g ml 1 chymostatin, 3 g ml 1 aprotinin, 1 mM PMSF and 100 U per ml RNase inhibitor) and centrifuged (12,000 g , 30 min, 4 C)
Patients often require multiple sessions over several weeks, which may increase the overall cost
Here we determined the rate constants for the individual redox reactions between the two redox-sensitive cysteinyl residues of roGFP2 and the active-site cysteinyl residues of the model class I glutaredoxin PfGrx from the malaria parasite Plasmodium falciparum using stopped-flow kinetic measurements in combination with redox mobility shift assays
J Pharm Biomed Anal 136:8191 Littlejohn EA, Monrad SU (2018) Early diagnosis and treatment of rheumatoid arthritis
doi: 10.1186/s13005-020-00234-2 18
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