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First, we filtered out low-quality reads, reads with adapter contamination, and reads with excessively high levels of unknown bases (N)

mPEG-MAL binds to every free Cys and alters the mobility of the protein on a non-reducing SDS-PA gel electrophoresis (PAGE) depending on how many thiol-mPEG conjugates are formed

Davidson TL, Chan K, Jarrard LE, Kanoski SE, Clegg DJ, Benoit SC (2009) Contributions of the hippocampus and medial prefrontal cortex to energy and body weight regulation