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In some cases, the most serious outcome has very few events, resulting in lower confidence results being used in pooled analysis, however the method is simpler and more transparent

Ruan J, Yao Y

Shang, Y., Song, N., He, R

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Additionally, most studies measure AMPK activity via single time-point assessments of p-AMPK Thr172, without quantifying downstream pathway dynamics (e.g., ACC phosphorylation, ULK1 activation) or tissue-specific AMPK isoform expressioncritical for understanding context-dependent regulation in NAFLD