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The retinal damage procedure included L-NAME administration in a total dose of 5 mg/kg, which was given by retrobulbar application in each eye, with 0.5 mg/0.1 ml saline per eye

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Cytotoxicity assessment The biocompatibility of the SHBG treatment was assessed with a resazurin-based test alamarBlue Cell Viability Reagent (DAL1100, Invitrogen, Massachusetts, USA)