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Primary neurons were identified using anti-MAP2 immunofluorescence.All cells were cultured in a 37 C and 5% CO 2 humidified incubator

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This small molecule, dihexa, penetrates the BBB, induces spinogenesis/synaptogenesis at picomolar concentrations [70] , and is slowly cleared from the blood (plasma stability t 1/2 = 335.5 min) [73]

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J Biol Chem 289:9952