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Briefly, 40 M tRNA fMet CAU , 600 mM MgCl 2 and 5 mM ClAc-L-Tyr-CME or ClAc-D-Tyr-CME in dimethylsulfoxide (DMSO) were mixed in 100 mM HEPES-KOH (pH 8.0) and incubated on ice for 1 h