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During this phase, type III collagen first deposited during proliferation is gradually replaced with stronger type I collagen

May also be mixed with water

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After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample

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