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Screening of GST fragments which can inhibit the GST activity 150 single positive clones (grown on the RGB plates), that could tightly bind to the glutathione Sepharose 4B, were picked up from the plates, and cultured separately in 50 ml of IMC medium containing 100 g/ml ampicillin for 15 hours at 25C, then induced with 100 g/ml tryptophan for 6 hours

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So far, therapeutic hepatitis B vaccines have been developed through different routes, including recombinant protein vaccines, DNA vaccines, and adenovirus vector vaccines

Pediatr Nephrol 27:12331247