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NCBI National Center for Biotechnology Information Search 1AQW: GLUTATHIONE S-TRANSFERASE IN COMPLEX WITH GLUTATHIONE PDB ID: 1AQW Download MMDB ID: 93022 PDB Deposition Date: 1997/8/3 Updated in MMDB: 2011/12 Experimental Method: x-ray diffraction Resolution: 1.8 Source Organism: Homo sapiens Similar Structures: VAST+ Download sequence data Biological Unit for 1AQW: dimeric

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Both animal and human data on dietary methionine and cardiovascular disease risk are too sparse to establish a methionine-excess involvement in atherothrombotic disease

They also noted that exercise increased glutathione concentrations among adults who led a sedentary lifestyle

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2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added