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By including a specific protease cleavage site between the GST tag and the recombinant protein, the tag can be cleaved off post-purification

The immediate release of glutamate after impact plays a significant role in this transient increase in glucose uptake (Kawamata et al., 1992)

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I also check that movement and strength in the hand and arm are intact, because that helps separate a minor sensory issue from anything involving the deeper nerves