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Arginine synthesis is impaired when small intestine and kidney function is impaired, thus creating a dietary requirement for arginine

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A total of 5 g His-UBE2m and 5 g GST-PRMT1 were added into a standard 50-L reaction mixture containing 5 l S-adenosyl-l-methionine (SAM, Amersham Bioscience), 50 mM Tris-HCl (pH 8.0), 20 mM KCl, 5 mM DTT, and 4 mM EDTA

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