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GCL is a heterodimeric enzyme composed of a glutamate-cysteine ligase catalytic subunit (GCLC), which confers enzymatic activity, and a glutamate-cysteine ligase modifier subunit (GCLM), which enhances GCLC efficiency and attenuates feedback inhibition by GSH

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Again, the 2-hydroxy 2-methyoxyestrone pathway is the most advantageous pathway for repairing DNA damage and reversing the effect of free-radicals, as well as glutathione activity

doi: 10.3389/fphar.2024.1330797 46 CarusoGDi PietroLCardaciVMaugeriSCaraciF

Metabolic analysis Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured with a 24-well XF Extracellular Flux Analyzer (Agilent Technologies) and Seahorse Wave Controller Software (Agilent Technologies), according to the manufacturers instructions

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