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In brief, 100 l of sample was incubated with 200 l MDA assay working solution for 15 min in a water bath at 100C

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Either the ventrogluteal muscle or the dorsogluteal muscle will work

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The resin is functionalized with an acid labile linker that allows for acidic cleavage usually trifluoroacetic acid, hydrofluoric acid (HF) or trifluoromethanesulfonic acid