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Among the cysteine residues in human Keap1, Cys 151, 273 and 288 are highly reactive and play an essential role for repression of Nrf2/ARE activation (Saito et al., 2016)

doi: 10.2460/ajvr.67.9.1557 14 IdaTMiyazatoMNaganobuKNakaharaKSatoMLinX-Zet al

Is the peptide being used for the same indication that was studied

You may have slight redness at the injection site for a day or two

Primary antibodies to DPEP-1 (Proteintech, #12222-1-AP, 1:1000), 4-HNE (Abcam, #ab46545, 1:1000), FTH1 (Abcam, #ab65080, 1:1000), GCLM (Abcam, #EPR6667, 1:1000), GCLC (Abcam, #EP13475, 1:2000), ZBP1 (Adipogen, #AG-20B-0010-c100, 1:1000), FSP1 (Santa Cruz, #sc-377120, 1:5000), and -actin (Cell Signaling, #3700S, 1:500) were diluted in skimmed milk (Carl Roth, #T145.2)

Only animals with no tumours at endpoint were excluded from analysis