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Pettorruso M, Martinotti G, Santacroce R, Montemitro C, Fanella F, di Giannantonio M

Cells were harvested, washed once with phosphate buffered saline (PBS, Gibco, #14190-094) and loosened from the cell flasks with Versene (Gibco, #15040-033) or TrypLE TM (Gibco, #12605-010)

This equalizes pressure and makes drawing the water much smoother

A standard starting dose in both men and women is 1mg daily

Its main goal is to influence important cellular processes related to aging, cellular senescence, and programmed cell death, also known as apoptosis